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It is one thing to record what is going on in a fly's brain if the insect's movement is restricted, but quite another to try to catch the light flash of brain cells from a fly that is walking around.
Each trial began with a light flash of 33 msec duration.
For each simulation we assumed that a light flash of a given strength was presented to the entire R layer.
B. atrophaeus growth was reduced by more than 4 log10 by 10 μM TMPyP and a single light flash of 10 or 20 J/cm.
In vivo degradation of type 1 CRYs does not require continuous illumination, and a single light flash of 1 ms duration leads to degradation of about 80% of Drosophila CRY in 60 min. Finally, we demonstrate that in contrast to animal type 2 CRYs and Arabidopsis CRY1 neither insect type 1 nor type 4 CRYs have autokinase activities.
Strikingly, we find that while a light flash of 1 ms duration is sufficient to cause nearly complete proteolysis of DmCRY and that DmCRY is subject to light-induced proteolysis in Drosophila, zebrafish, and mammalian cell lines, ZfCRY4 does not undergo light-induced proteolysis by flash or continuous illumination in either orthologous or heterologous hosts.
Similar(54)
After an additional 5 min of dark adaptation, rod response was measured by averaging 4 dim light flashes of 80 cd.m−2×0.12 msec at >2 sec interval.
TKO mice and wild type controls were dark-adapted for >12 hrs and exposed to white light flashes of increasing irradiance (−4.5 to 3.5 log10 cd/m2).
To explore the function of SV2B, we compared neurotransmission in the retinas of wild-type and SV2B knockout mice by measuring the electroretinographic response to light flashes of varying intensity [22].
Stimulation of larvae with light flashes of 0.5 s (intensity: 207 W/m).
The functionality of the cell was confirmed by regular observations of the amplitude of the cell response (typically of 20-25 pA) to brief light flashes of saturating intensity.
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