Sentence examples for light fixation from inspiring English sources

Surface Eag1 immunolabeling was performed after light fixation (0.1% PFA for 10 min at room temperature) by incubating the cells for 1 hour at room temperature with the indicated antibody concentrations in Ringer and 5% of Donkey serum.

For the adult fish, tumors or corresponding healty skin from control fish was excised after a light fixation and maintained in 4% paraformaldehyde overnight, before equilibration in 25% sucrose in phosphate buffered saline (PBS) and embedding in OCT.

While preserving the cells and allowing for storage and access to multiple plates at any one time, light fixation with formalin allows preservation of potentially-antigenic epitopes on the surface of target cells.

This method allows for an adequate diffusion of the antibody but it decreases the preservation of the material due to light fixation, and, therefore, it decreases the quality of the image [16].

Light fixation of frozen brain sections with 10% acetone completely ablated LRRK2 signal for all antibodies.

During high-throughput screening, light fixation using 0.5% paraformaldehyde was performed for 5 min prior to two additional washes with PBS and a FLiPR plate reader was used.

Once participants reported a lateralized phosphene, they were asked to fixate a dimly lit fixation point in the centre of the monitor and to point the curser in the middle of the phosphene.

In these difference maps, area DP has more reflected light during fixation in the upper part of the visual field indicating an increased neuronal activity with upper eye positions, as expected based on prior studies [5], [14].

A small light blue fixation cross remained in the center of the screen throughout the total scanning time, and participants were instructed to fixate the cross during the whole scan session.

The tissue specimens were processed routinely for light microscopy (fixation, dehydrating, embedding, and sectioning) and staining with hematoxylin and eosin (HE, basic staining) and van Gieson's stain (VG, nonspecific collagen staining).

Each trial started by lighting a fixation LED at the center.