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All the dark and photochemical (light) experiments were conducted in a 200 mL water-jacketed beaker to prevent suspension heating during the 8 h experiments.
"Low light" experiments were conducted in a Sunbox fitted with blue theater gels (Roscolux #69, Rosco, Stamford, CT) and set to produce a noon maximum of about 40 µmol quanta m−2 s−1; "high light" experiments used unfiltered white light with a noon maximum of about 250 µmol quanta m−2 s−1.
Fluorescent light experiments were replicated in two different lines: vs ; Sudbury; Sudbury and vs ; Rum Cay; Rum Cay.
In this light, experiments were performed in which the p300/CREB binding protein-associated factor (PCAF) activator pentadecylidenemalonate 1b (SPV-106), a HAT activator, was directly applied on wounded mouse skin.
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While interpretation of this comparison cannot be conclusive because the integrated 24 hour photon flux in the continuous light experiments was 35 60% that in the diel experiment, and the cells were growing at lower growth rates, it is striking that "stress-related" genes constitute a significant fraction of the overrepresented transcripts under continuous light conditions.
Vitrified somatic embryos obtained in the 'LED light experiment' were cut into tiny pieces forming an embryogenic mass.
For further growth, small plantlets developed in the 'LED light experiment' were transferred to glass bottles (650 mL capacity) each containing 100 mL of MS basal medium supplemented with 3%% sucrose and 0.4 % GPP and incubated in a culture room at 25 ± 2 °C, a light and dark cycle of 16/8 h and an illumination intensity of 34 μmol/m2 s.
The 60 μmol photons m-2 s-1 continuous light experiment was completed prior to the other two, which were run simultaneously in separate incubators.
A lighting experiment is conducted to identify the gap between comfortable and stimulating illuminance settings.
Ultraviolet (UV) light illumination experiments were carried out at room temperature (25°C) with a light density 2 mW/cm2 using an UV lamp (GL15, UV-C; Sankyo Denki Co., Kanagawa, Japan).
Since it is known that the psbA2 promoter has a higher activity at high light, the experiments were repeated for a subset of the 13R-MO producing strains at high light (100 μE).
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