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Torch light examination was performed to assess cataract and anterior segment pathology only if the subject had presenting VA < 6/18 in either eye.
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Wood's light examination is of little value nowadays because in western countries most infections are due to T. tonsurans, which does not fluoresce.
Light microscopic examination was performed using a Leica DMLB microscope.
Light microscopy examination was performed on samples stained by H & E, PAS, Jones and Masson's trichrome.
For the assessment of TUNEL- and Ki-67-positive cells, light microscopic examination was performed at a magnification of ×400 with the use of a counting grid.
Light microscopy examination was performed by three different assessors, blinded to the metastatic outcome of these tumours or identity of the primary antibody.
After administration of one drop of 0.4% oxybuprocaine and one drop of a lubricant gel (0.2% carbomer), the patient was asked to fixate on a small, bright, red light as the examination was performed in the contralateral eye.
Semithin (1 µm) cross sections for light microscopic examination were cut with a diamond knife on an ultramicrotome.
Kidney histology examination was by light microscopy only.
The pseudogaster contents were analysed, and a histopathological examination was performed using light and electron microscopy.
Slide examination was performed with light microscope at ×1000 magnification and the AFBs identified were graded according to the International Union against Tuberculosis and Lung Disease IUATLDD) and the WHO smear grading scale.
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