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However, in light deletion of gna1 caused enhanced growth under conditions of oxidative stress, whereas constitutive activation of GNA1 resulted in decreased growth under these conditions.
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Light microscopy of deletion strains.
Genetic alterations are color-coded with red indicating amplification; light blue, homozygous deletion; green box, somatic mutation; pink frame, mRNA upregulation; and blue frame, mRNA downregulation.
All strains used are lysine auxotrophs and were grown in the presence of either heavy L-lysine (wt cells) or light L-lysine (deletion mutants).
In this light, the mtDNA deletions in the human TM that accumulate during the aging process are the result of this reduced capability.
In light, however the deletion-phenotype of Δ env1 was observed with respect to growth, conidiation and cellulase gene transcription.
This idea is supported by the observation that protein disorder is common in insertions and deletions (Light et al., 2013).
However, this epistatic effect of env1 deletion was light dependent: both GNA1QLE and GNA3QLE showed the same phenotype as GNA1QL and GNA3QL in darkness, while the Δ env1 mutant shows wild-type behaviour in darkness.
Additional file 1: Figure S2 shows light microscopy images of the deletion strains.
To study the influence of isiA deletion on the light usage of both the whole-chain and the PSII- mediated electron transport activities, we cultured the wild type and isiA- strains in BG-11 under LL conditions.
A third of these loci corresponded to a single indel in one single genome, typically a strain-specific deletion (indicated by light colors and a large number of families with low diversity in fig. 10).
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