Yet, in a city where traffic rules used to be viewed as entirely optional, a dozen pedestrians all stayed rooted on the sidewalk, waiting obediently in the rain as a red light counted down the 160 seconds they needed to wait before crossing.
Values were standardized to the amount of protein, and photons of light counted were expressed as RLU per microgram of protein.
They are looking down, or looking at their manager, or looking out into the grandstand trying to count the house, or they are peering into the ring lights counting the moths".
You begin in the dimly lit counting house of a manufacturing firm, in a small corner furnished with a single, wooden, high-backed, cubby-holed Bob Cratchit desk, circa 1840.
The concept is simple: Quitbit, a high-tech USB lighter, counts every time you use it, and then tallies it up on a nifty iPhone app.
Brown et al. has published utility values for minimal light perception, light perception, counting fingers and reading letters.
In various modern master clocks the pendulum operates a light count wheel that turns through the pitch of one tooth every double swing and is arranged to release a lever every half minute.
The luciferase activity given in light counts per second was normalized against the protein concentration in each sample and given as relative light units (RLU).
The obtained luciferase activities were normalised to the protein amount and calculated as light count per second per µg protein (lcps/µg protein) and relative luciferase units (RLU) were compared to un-stimulated controls that were set to 1.
Scattered light count rates exponentially increase as nucleation and assembly of micelles occur.
Luciferase activity was monitored as per the instructions provided in the kit supplied by Promega and the light counts were recorded using a luminometer (LUMAC Biocounter M2000).
