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After 15 minutes of incubation away from light, cells were directly analyzed by FACScan and evaluated by the CellQuest program.
After shaking for 30 min in light, cells were washed twice with TAP medium.
When transplanted in immunodeficient mice, light cells were highly tumorigenic compared with bulk MCF7 cells.
Equivalent amounts of heavy and light cells were mixed 1 1 before proceeding with a proteasome affinity purification.
WT cells were grown under rich conditions with heavy C6N2-lysine or light C6N2-lysine, and light cells were subsequently starved for 3 hr.
Dark cells were found to be the predominant type in TEBs, intermediate cells and myoepithelial cells were present in significantly lower percentages, and light cells were only occasionally seen so their percentage was combined with that of intermediate cells.
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The cell type with the Htr preference (shown as the light cells) was always located near the center of the aggregate and was surrounded by the cell type with the Hm preference (shown as the dark cells).
For light microscopy, cells were imaged using a Nikon Eclipse TS100 light microscope ×40 objective and a Canon EOS 7D digital camera.
High light (HL) cells were spotted onto BG-11 plates and illuminated for 10 min under 600 μmol m 2 s–1 white light.
Light labeled cells were arrested in G0 phase while medium and heavy labeled cells were arrested in G2 and G1S phases, respectively.
Because only the neurons involved in the mouse's experience of this chamber became sensitive to light, these cells were essentially labeled for later reactivation.
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