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The product fraction was additionally purified using a SepPak Alumina N light cartridge.
After purification via one Sep-Pak C8 light cartridge, the ethanolic product fraction was diluted with PBS and used as such for the experiments.
18F-fluoride was trapped with an ion-exchange resin QMA sep-pak light cartridge and eluted from the resin by 210 mmol/L K2CO3 solution (0.2 mL) and 31 mg/mL Kryptofix 2.2.2 in acetonitrile solution (K222, 0.7 mL).
Briefly, once transferred into a dedicated (ventilated and lead-shielded) hot cell, [18F] fluoride was first trapped on a preconditioned Sep-Pak® Accell Plus QMA Light cartridge (Waters, Milford, MA, USA) and subsequently eluted from the cartridge with a solution of Kryptofix K2.2.2/K2CO3 in a mixture of water and acetonitrile.
The radiotracer was isolated by semi-preparative HPLC (Reprosil-Pur AQ column, 250×10mm, 46 % ACN/aqu. 20 mM NH4OAc, flow 5.5 ml/min) followed by purification with a Sep-Pak C18 Plus light cartridge and formulation in isotonic saline containing 10% ethanol.
The Gd6 LGa complex was purified by passing the mixture through a preconditioned Sep-Pak C-18 light cartridge.
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The Sep-Pak C18 Plus Light Cartridges (Waters) were used for desalting (parameters: 130 mg sorbent per cartridge, 55 105 μm particle size, 50/pk).
SepPak C18 Plus Light cartridges (Waters Corp., Milford, MA, USA) were used for desalting (parameters: 130 mg sorbent per cartridge, 55 105 μm particle size).
Chromafix 30-PS-HCO3F separation cartridges were obtained from GE Medical Systems and C-18 SepPak (light) cartridges were obtained from Waters Corp. (Milford, MA).
He recounts a charming episode, for instance, in which George Washington and Thomas Paine floated together one night down a New Jersey creek, lighting cartridge paper at the water's surface to determine whose theory was correct about the source of swamp gas.
After heating to 60 °C for 10 min, the reaction mixture was allowed to cool to RT, and [64Cu]NOTA-pentixather was isolated via solid phase extraction using a C8-light cartridge (Waters).
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