RCM provides real-time minimally invasive 3-D sectioning of in vivo (living) or ex vivo (newly biopsied) individual cells and tissues using variations in the optical properties of the natural backscattering of light from different cellular and subcellular structures without the use of labeling cells fluorescently or otherwise [ 10, 11].
To determine the relationship between light microscopic types of PrPd and subcellular sites of PrPd accumulation, individual patterns were identified by light microscopy in 1-µm-thick immunolabeled sections and then located in serial sections by electron microscopy.
In order to clarify the compartment specific importance of ascorbate and glutathione in the protection against high light conditions, subcellular ascorbate and glutathione contents were also evaluated in mutants deficient in ascorbate and glutathione contents.
The CCDB was formally launched in 2002 as an on-line repository of high-resolution 3D light and electron microscopic reconstructions of cells and subcellular structures.
Similarly useful databases include CCDB (ccdb.ucsd.edu), which provides a venue for sharing and mining cellular and subcellular data derived from light and electron microscopy, including correlated imaging.
However, their application within cells is limited by impaired disulfide bond formation in the reducing environment of the cytoplasm, inefficient assembly of the epitope-recognizing parts of the variable light and heavy chains, and difficult subcellular targeting [ 12, 13].
Research on these molecules as agents in tumor progression is fundamental and brings to light the intricate, complex relationships occurring at cellular and subcellular levels.
The dynamic and subcellular visualization enabled by FRET can shed new light on the molecular mechanisms regulating integrin signaling and advance our knowledge in the understanding of integrin‐related pathophysiological processes.
The goal of the present study was to examine GSK3β distribution and subcellular localization in the brain using immunohistochemistry combined with light and electron microscopy.
Light dependent changes in DNA binding activity of several plant bZIP transcription factors have been shown to involve both phosphorylation and subcellular translocation from cytoplasm to nucleus [ 38].
