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For the nighttime measurements, larvae were quickly mounted under a dim red light and imaging was performed in complete darkness.
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In order to reduce the effects of light source, both illumination characteristics of light source and imaging characteristics of camera have been designed experimentally to obtain acceptable facial image.
Given the cost and technical challenges connected with extending the bandwidth of light sources and imaging systems, it becomes increasingly important to determine the optimal specifications early during the design process.
Then a series of system design criteria are illustrated accordingly, and by following them, three hardware modules of the imaging system, including illuminant, lighting path and imaging camera, are optimally proposed.
To speed its development, about 40% of the vehicle's parts, including the robotic arms, underwater lighting equipment, and imaging system, were imported or made abroad, project chief designer Xu Qinan told Chinese media.
Recent technical developments in broadband optical light sources, detectors and imaging processing methods have boosted its imaging ability in terms of resolution and speed, permitting rapid volumetric scanning of tissue microstructures in vivo [ 9– 13].
Many of the problems relate to their small size, which is less than the wavelength of visible light and hampers imaging by light microscopy.[ 8] For example, while the standard method of production (extrusion through a microporous filter) allows control over size, a range of diameters are always present in a given sample.
The images, acquired using the same lighting and imaging parameters, were adjusted to the same threshold and a histogram was produced for each picture.
The wavefront sensing and imaging light sources are diode-pumped solid-state (DPSS) lasers (Altechna, Vilnius, Lithuania) with nominal centre wavelengths of 670 and 532 nm, respectively.
The optimization of FMI for whole-body three-dimensional imaging in small animals is contingent upon developments in three distinct categories: fluorescent probe design, light propagation modeling through tissue, and imaging system design [ 3].
The head of the imaging system contains the optical components (light source and imaging optics) as well as a computer-controlled linear translation stage for sequential scanning of the area of interest.
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