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Freeze-dried CNPs (∼5 mg) were resuspended in 1 ml of phosphate-buffered saline solution (PBS, pH 7.4) and incubated at 37°C under light agitation of 150 rpm using an incubator shaker (Wadegati Labequip, India; n = 3).
"The morphologies of hand-blown glass vessels function both as lighting and heating elements for the human occupants, and high functioning photobioreactors which provide heat, light, agitation, air supply, nutrient and waste control to the living algae inside," Douenias and Frier explain in their press release.
Primary antibody incubation was done for 48 h at 4 °C with light agitation.
After washing, samples were blocked (3% BSA, 0.05% saponine) for 1 h at room temperature and incubated with cytochrome c antibody overnight at 4 °C with light agitation.
If the vent feathers are matted with poo, you may need to soak for a few minutes to dissolve it with light agitation of the water.
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Ancoli-Israel, S. et al. Effect of light on agitation in institutionalized patients with severe Alzheimer disease.
Photofermentative hydrogen production is challenged by the photobioreactor design that can overcome poor light penetration, agitation and temperature control.
Temperature, light and agitation conditions were the same as for the stock solutions preparation.
Ten-day-old conidia were inoculated into a 50-mL medium at 10 conidia/mL final concentrations and grown at 25°C in constant light and agitation (200 rpm).
For induction experiments, axenically grown seedlings were transferred to liquid medium and incubated in the light with agitation at 100 rpm for 6 h.
Such a condition is suitable for algal species that cannot grow in complete darkness but require low light or agitation [ 1].
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