The left inferior peritoneal space was then opened and a silk ligature tied around the iliac artery inferior to the uterine artery takeoff, typically just inferior to the hypogastric trunk artery.
There was no rupture or leakage from the arteries due to ligature tying or pressure testing.
For the elastomeric ligatures, tying a needle holder was used, and after insertion of the elastic, a 3-min waiting period before the tests was determined, to enable initial relaxation of the material, as recommended in other studies [28, 29].
In Group B, each artery was ligated with three circumferential ligatures tied with a sliding knot with two overthrows.
Leaking pressure for ligatures tied with monofilament suture material was 1050.0 mmHg (750-1050) for surgeon's knots (Subgroup A-M) and 1050.00 (750-1050) mmHg for sliding knots (Subgroup B-M).
She told the court analysis of one of these - the belt from Helen's raincoat that was found around her wrists - suggested the two girls may have still been together when the ligature was tied.
The ligature was tied around the vein over the top of the guide wire.
Briefly, a ligature was tied around the proximal duodenum to isolate the stomach, and the forestomach was discarded.
In brief, after a left-sided thoracotomy in the second intercostal space, a 7-0 siligatureure was tied around the transverse aorta and a 27-gauge blunted needle that was subsequently removed (Movie I in the online-only Data Supplement), whereas, in sham-operated animals, the ligature was tied loosely around the aorta.
In order to allow for an isolated perfusion of the left kidney, a third ligature was tied around the right renal artery and vein to prevent perfusion of the right kidney, just prior to insertion of the perfusion catheter.
The brackets and wires were cleaned with alcohol wipes before the module or ligatures were tied to form a test unit.
