Sentence examples for ligation kit from inspiring English sources

This vector was digested with HindIII restriction enzyme and ligated using a ligation kit (Gibco-BRL, Grand Island, NY, USA).

Paired-end sequencing adapters (Illumina) were ligated with the Quick Ligation kit (New England Biolabs).

One microgram of genomic DNA of CrV was digested with the restriction enzyme Hind III and self-ligated using a Takara ligation kit version 3.0 (Takara Bio).

The amplified product fragment was then digested using SpeI and SphI and ligated using NEB quick ligation kit to the SphI/SpeI 1,994-bp 1,994-bp fragment82 to generate pRfrom

Digested vector and amplified 3′UTRs were ligated using a Rapid DNA ligation kit (Roche Diagnostics, Basel, Switzerland).

Both insert and vector were gel purified using the Qiagen Gel extraction kit, then ligated using a Rapid DNA Ligation kit (Roche, Branchburg, NJ, USA).

The PCR products and the open plasmid were ligated using the Roche Rapid DNA Ligation Kit.

Annealed oligos were ligated into pSM30 vector using Rapid Ligation Kit (Roche) with 15:1 insert:vector ratio.

After A-tailing using exo-Klenow fragment (New England Biolabs, Ipswich, Massachusetts, NEB M0212L), barcoded adaptors were ligated with Epicentre Fast-Link DNA Ligation Kit (Epicentre LK6201H).

Experimental DNA fragments were ligated to linker primers using the FastLink DNA ligation kit (Epicentre, Madison, WI, USA).

Divide the ligation mix into 20 μl portions in Ready-to-Go-ligation kit tubes (Amersham Biosciences).2) Incubate at 16°C for 3h to over night.