Sentence examples for lifting cells from inspiring English sources

Because of the anatomical separation of the MOE and AOE, we were absolutely sure that we were lifting cells from the appropriate epithelium.

However, it is important to note that lifting cells from dishes and other routine cell handling steps often results in PM wounding and Ca2+ influx.

To lift cells, 0.05% Trypsin (HyClone) was used.

Briefly, culture medium was collected and centrifuged at 800×g for 5 min and additional 2,000×g for 10 min to remove lifted cells.

Pipette up and down ten times to lift cells and ensure single cell suspension.

Kinetics of current responses recorded from excised patches and from lifted cells did not show any clear difference and were pooled together.

All cell lines were grown at 37°C, in a 5% (w/v) CO2 incubator and were passaged when confluent, usually twice per week using EDTA to lift cells from the monolayers.

However, in the excised patch mode, currents elicited by 30 µ m GABA (or lower) were often too small to reliably quantify their time course (especially onset kinetics) and, for this GABA concentration range, recordings were preferentially made in the whole-cell configuration selecting cells with small diameter and capacitance of less than 8 pF (small lifted cells).

For control experiments a piezo translator-driven double-barrelled application pipette was used to expose the lifted cell either to 5-HT-free or 5-HT-containing solution.

With onset of VSG ES reactivation, the G1-retardation was lifted, cell cycle progression was accelerated, and at day 10, the population doubling times were back to normal (6 hr).

Following lifting the cells with 2 mM EDTA in PBS, pH 7.4, the remaining matrix was incubated with 0.1% deoxycholate in 2 mM EDTA in PBS, pH 7.4 for 10 min.