You can imagine a lifetime of interactions like this convincing someone that, if even his adoring family sees him as somehow "other," he had no chance pleading his case to police.
In the AHA guidelines for primary prevention of CVD and stroke, 25 a panel of physicians summarised, "Primary prevention, by its very nature, requires a lifetime of interactions that virtually define successful provider-patient relationships".
Sensitivity can be retained if measurements are performed on the same pMHC due to serial rebinding 21, but this will result in a trade-off between specificity and speed 14, 22. Indeed, if the intrinsic lifetime of interactions between a TCR and cognate pMHC is of the order of 10 s 10, performing multiple lifetime determinations on a single TCR/pMHC couple may require a minute or more.
This difference in apparent outcome is not exclusive to particular pairings of experimental methods; it is a direct consequence of the time scale accessible to a method that is, any method that probes an interaction at a rate longer than the lifetime of the interaction will miss it, whereas other methods that can sample faster or artificially stabilize the interaction will observe it.
Most of us assume that honesty and generosity are personality traits polished over a lifetime of social interaction.
Therefore, the formation of membrane tethers between the pathogens and host cells may substantially increase the lifetime of the interaction, thus favoring host colonization.
11 An early finding was that the capacity of a pMHC to activate a T cell is positively correlated to the lifetime of TCR/pMHC interaction.
14 Therefore, there is a strong interest in obtaining quantitative information on the movements of cell membranes with a time resolution within the second range, corresponding to the typical lifetime of TCR/pMHC interaction, and a distance resolution close to the length of the TCR/pMHC couple, which is about 15 nm.
Much evidence suggests that there is a deep link between the lifetime of molecular interactions between T cell receptors and ligands and T cell activation, but the precise mechanisms of bond formation and dissociation remain incompletely understood.
Binding under these conditions was sensitive to ionic strength with on-rate and lifetime of motor microtubule interactions decreasing as the salt concentration was raised from 30 mM to 100 mM KCl (not shown).
