Exact(41)
Lifespan assay were conducted as described previously [15].
Day 1 of the lifespan assay started 68 h after seeding the animals into plates.
The day of the shift is counted as day 0 in the adult lifespan assay.
These results are consistent with our data, which show a striking effect by sex in the lifespan assay.
To prevent this undesirable effectin our lifespan assay, we used FUDR treatment of young adult worms to render them sterile.
To rule out the possibility that the lifespan change may result from potential variation in genetic background among fly cohorts, lifespan assay for control flies with and without the Atg1Δ3D allele was also performed in parallel with Aβ1 42 flies.
Similar(18)
The lifespan assays were carried out in CL2006 at 16 °C.
Live animals were scored the same way as in lifespan assays.
Briefly, for each experiment, at least 50 synchronized L4 larvae or young adult worms were treated and maintained as described in the lifespan assays.
Animals were raised from eggs similar to the lifespan assays.
All other lifespan assays were conducted at 20°C.
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