300ng of total RNA for half life arrays, or 100ng for immunoprecipitation arrays, were used to generate labeled cDNA fragments for hybridization to Mouse Affymetrix Gene 1.0 ST Arrays following the manufacturer's protocol (GeneChip™ WT cDNA Synthesis Kit #900652 and #900720).
The whole-life array revealed exclusive miR-29 expression at the early 1st instar and late 1st molt larval stages.
The whole-life array revealed that, in contrast to miR-29b, miR-275 expression peaked in early female pupae, but not in the early female moth.
Twenty unique miRNAs were highly expressed, as shown by microarray experiments, during the early larval stages, including 15 strongly detected miRNAs identified in the whole-life array (Additional file 6).
The miRNA profiles obtained with the general whole-life array provide an insight into the stage-specific miRNA transcriptome, and may thus facilitate the identification of sequential classes of miRNAs and their primary targets associated with distinct developmental stages.
Only one time-point (day 6 embryo) was examined using the whole-life array, and this did not provide sufficient information on expression profiles in developing embryos of silkworm.
Leader includes, in his account of Bellow's life, an array of conflicts and foibles that don't depart drastically from the run of modern lives not lived on the literary grid.
There won't be a "normal" woman's body, just a life-affirming array of every type of female body under the sun – boobs of all shapes and sizes, curvy hips, slim hips, broad shoulders, tiny bums, huge powerful gluteal asses, wispy legs that travel for miles, hulking great thighs that accelerate over 100m, bellies that dance or are neatly stacked in six-pack squares.
Many different approaches have been suggested, such as appropriate immobilization of cells or addition of particular additives to reduce stress factors; however, other innovative methods to significantly extend the shelf life of arrays are required, particularly for commercialization in industry.
Many of us go through life with an array of undone tasks, large and small, nibbling at our conscience.
Musharraf faces serious threats to his life from an array of dangerous militants, and he deserves adequate protection.
