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Amplification of a coding DyP-type peroxidase sequence of P. sapidus from the cDNA library was performed by PCR.
Prior to sequencing, the preparation of the DNA library was performed using a Template Preparation Kit (Pacific Biosciences, Inc., CA) with fragments size targeted at 10 kb.
Screening of the Schistosoma library was performed by using the yeast-mating technique.
Normalization of the primary library was performed as previously described by Bonaldo and co-workers [218].
Screening of the phage library was performed according to the method described by Smith [39].
Normalization of the cDNA library was performed to compensate for very abundant transcripts, thereby increasing the odds of sequencing rare transcripts.
To identify protein interaction partners capable of regulating the GLTP-induced changes in cell shape, yeast two-hybrid screening of a human brain cDNA library was performed.
Screening of the library was performed essentially following instructions for the ProQuest two-hybrid system (Life Technologies) and has been previously described [18].
Construction of the library was performed in two steps.
Sequencing of the library was performed using Illumina HiSeq™ 2000.
High-throughput sequencing of this library was performed subsequently.
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