Sentence examples for library transformation from inspiring English sources

We also used a second strategy, where selection pressure was applied directly after library transformation, by plating the transformed and recovered cells in a 1-hexene saturated atmosphere.

The high proportion of phenotypic deviations and the wide range of abnormalities observed among the transformants suggests that mutagenesis by gene-disruption library transformation is a useful strategy to establish a highly diverse population of Physcomitrella patens mutants for functional genome analysis.

Taken together, the collection of Physcomitrella transformants that we are establishing by a cDNA based gene-disruption library transformation approach shows a high degree of phenotypic diversity.

To assess the diversity of the libraries, bacterial colonies from library transformation plates were analyzed by colony PCR and sequencing.

One yeast colony per library transformation was re-streaked onto fresh plates and subsequently grown in culture medium lacking uracil to enrich for the ARS bearing plasmid.

After library transformation, cells are plated on minimal synthetic dropout non-induction medium (Sigma) that selects for both the bait (PIG-2) and the AD/library plasmids to improve the chances of detecting AD fusion proteins.

Vector only control transformations were done in parallel with the library transformations.

Freshly prepared electrocompetent yeast were used for most library transformations, and the remainder was frozen at -80°C for use in low efficiency transformations.

In Brent, the move is being sold to the public as the "Libraries Transformation Project".

Therefore, we initially introduced the corresponding archive clone (ASKA library) by transformation and then propagated P1 from the resulting transformants.

Coverage of the ARS library before transformation was determined to be 776 ± 11 for ARS_L38.