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READING MATERIAL: The Larchmont library stocks books at the local station.
Transformed clone library stocks were sequenced by Agencourt Bioscience.
Plasmid library stocks were obtained and plasmid DNA prepared using the Qiagen plasmid purification kit.
All library stocks were made up in 1x Dharmacon Buffer (20 mM KCl, 6 mM HEPES-pH 7.5, 0.2 mM MgCl2), transferred to twin.tec 384-well PCR plates (Eppendorf), sealed with PlateLoc Thermal Seals (Velocity-11), and stored at −20C.
Primary titres were determined by growth assays of serially-diluted library stocks.
These compounds were dissolved in 100% dimethyl sulfoxide (DMSO) to generate library stocks with a final a concentration of 2 mg/ml.
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