For practically accessible library sizes, probe lengths cannot be easily extended beyond 10 nt [13].
Different library sizes show difference in N50 values while the smallest library size (Lib_300) always gives the lowest N50 value.
These factors are used for calculating effective library sizes, i.e., library sizes multiplied by the TMM factors.
The remaining two combinations (DESeq/TbT and baySeq/TbT) introduced the effective library sizes, i.e., the original library sizes multiplied by the TbT factors.
First, we estimated effective library sizes as the median expression estimates across all genes.
The library sizes obtained for the seven samples are approximately 7,400,000.
The library sizes ranged from approximately 120,000 reads to over 1.2 M.
Plating an aliquot of the library and counting the colony number determined the library size.
One criterion which was not used for library ranking is library size.
These methods include full, partial and random enumerations as well as calculation of the library size.
A typical in vitro system, ribosome display, provides the largest available library size.
