Exact(2)
However, PCR amplification may introduce bias in the form of non-uniform distribution of reads; it can introduce artefacts into the library prepared for sequencing [ 25].
For each individual, a library prepared for the Illumina GA2 platform was split into two portions, with one portion sequenced at the Argonne sequencing centre and the other at the Yale sequencing centre.
Similar(58)
He loved history and books of all kinds, really, and spent most of his free time in the library, preparing for his debating-team competitions.
The sequencing libraries prepared for this work were obtained by combining 50 µL of sheared DNA with 40 µL of Agencourt AMPure XP SPRI magnetic beads and incubated at room temperature for 5 7 minutes.
Tissue samples per block (4 subsamples) were pooled for a total of 8 libraries prepared for each tissue (16 libraries total).
Details of Sequencing Quality control of DNA libraries prepared for sequencing was made by qPCR and by running the products on a Bioanalyzer (BioRad).
Libraries prepared for Varuna and Heera were sequenced as 2×101 nt paired end reads on a Genome Analyzer IIx instrument (Illumina Inc, San Diego, USA).
Combining the data from these libraries with the data from libraries prepared for protocol development (n = 3), our dataset consists of ~600 million mapped strand-specific reads derived from 11 libraries.
One hundred indexed mRNA libraries (50 blood monocyte libraries and 50 milk monocyte libraries) were prepared for cluster generation using TruSeq v2 RNA sample preparation kits (Illumina, San Diego, CA).
A parametrized diffusion cross section library was prepared for the HPLWR assembly with the MULTICELL neutronic transport code.
A saturation mutagenesis library was prepared for each residue (Tyr110 and Asp111), and activity of the resulting variants was assayed on different chain length substrates.
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