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The result is a library of trees representing a large variety of differently sized random gene partitions.
Moreover, RADICAL monitors the dynamics of concatenation by calculating support statistics for candidate test topologies assessed against the library of trees.
Using this approach, the dynamics of concatenation was monitored by calculating support statistics for candidate test topologies assessed against the library of trees.
RADICAL generates a library of trees along a set of random concatenation chains varying from one gene to whole-matrix concatenation.
Here, we introduce a technique called Random Addition Concatenation Analysis (RADICAL), a method that generates a library of trees along a set of random concatenation chains varying from one gene to whole-genome concatenation.
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In this study, enriched DNA libraries of tree peony, a well-known ornamental woody shrub, were used for high-throughput microsatellite development by 454 GS-FLX Titanium pyrosequencing.
At select points along these chains, RADICAL calculates trees representing the data concatenated to that point, creating a library of phylogenetic trees.
In the current study, we used the Roche 454 GS-FLX Titanium pyrosequencing platform to obtain a comprehensive transcriptome of P. tabuliformis from normalised cDNA libraries of adult trees (xylem, phloem, vascular cambium, needles, cones and strobili).
Based on this representation, spin image features are extracted that comprise only those components of highest variability among a given set of library trees.
Premise of the study: We developed and characterized five polymorphic microsatellite loci from an AC-enriched genomic library of a rare tree, Tapiscia sinensis, and six compound microsatellite primers using a dual-suppression-PCR based approach, to investigate geographical genetic structure.
For this purpose, cDNA libraries from the leaves of trees in "on year" and in "off year" in July (when fruits start to appear) and in November (harvest time) were constructed.
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