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These leaf cells are generally implemented in a full-custom design flow as they are optimized to form the foundation or library of cells that are used repeatedly to build an entire design.
The library of cells was challenged with 70 pg ml−1 TcdB for 8 h before the loosely attached round cells were removed by repeated pipetting.
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This is based on a library of cell clones, each expressing a different fluorescently tagged protein expressed from its natural chromosomal location and under its native regulation.
Having established that our library of cell lines exhibited variation in both Prx2 mRNA and protein levels in response to the silencing of a variety of components within the redox regulatory network, we next asked if it was possible to modulate non-specific protein levels by shRNA-induced gradations in one of the redox couples.
Survival-based reporters, such as HIS3, provide selective pressure, thereby enabling rapid analysis of large libraries of cells.
The cDNA database is based on libraries of cells obtained from several different stages of the developmental cycle of Dictyostelium [ 23].
Libraries of cells that contain RNAi transgenes that target mRNAs from random regions of the genome can also be used in conjunction with high-throughput sequencing approaches to screen RNAi knockdown effects on a genome-wide level.
The normalized cDNA libraries of cells grown under the above conditions were pooled and sequenced using the 454 GS FLX Titanium, and the D. tertiolecta transcriptome was assembled from the resulting sequencing reads.
In 2003, we began cell-based screening experiments involving a combinatorial libraries of cell permeant, organelle-targeted fluorescent compounds [25].
ThruPLEX Plasma-seq's single-tube workflow is easily transferred to the Biomek FXP Workstation to enable high-throughput automated preparation of Illumina next-generation sequencing (NGS) libraries of cell-free DNA for liquid biopsies.
To facilitate routine conduct of such testing, libraries of cell lines from many different subgroups (infants, the elderly, asthmatics, diabetics, etc).
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