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The λ-Express Predigested Vector and ZAP Express Predigested Gigapack cloning kits (BamH1/CIAP-treated) were used for the construction of a λ-phage metagenome library of a sample taken from a pilot-plant biogas reactor, as described by the manufacturer (Stratagene).
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Two independent single-end cDNA libraries of sample A were constructed and sequenced, yielding datasets 'A' that contained ~29.7 million reads and 'A-rep' that was sequenced at 3-fold lower depth and contained ~9 million reads.
Subsequently, the reads from all libraries of a particular sample were combined.
We constructed a metagenomic library from a sample of Baltic Sea water and identified a gene encoding a novel enzyme with β-galactosidase, β-fucosidase and β-glucosidase activities.
PacBio library construction A sample of 20 μg pure and high-molecular-weight DNA is required to prepare size- selected approximately 20 kb SMRTbell templates.
He has a library of textile sample books, but prefers that customers show up with their fabric in hand (with measurements and a digital photo, he can provide yardage estimates over the phone or via e-mail), and ideas about how the finished piece will look.
Then, an mRNA of each sample was extracted, a cDNA library of each sample was constructed, and Illumina sequencing was carried out as described above.
To validate the library, a sample of 97 embryos has been screened for determining the number of embryos lacking SE labelling.
The cDNA library of each sample was loaded to a single lane of an Illumina flow cell.
Armed with an Elektron Monomachine, a library of samples, and an aesthetic sensibility shaped as much by family history and personal experiences as by late 80s video games and Brooklyn electronic music, Takahashi has crafted NoGeo (a pun on the 80s arcade game manufacturer Neo Geo) into a poem-like dance album.
To measure public library use in a sample of families with young children and examine associations with reading aloud.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com