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From the barcoded library, manual emulsion PCR was performed, followed by sequencing eight multiplexed samples using Ion 318 chips and the IT-PGM Torrent Suite v2.0 (Life Technologies) was used for analysis.
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We conducted a literature search using PsycINFO, PSYNDEX, MEDLINE, EMBASE, PQDT OPEN, WorldCat, Cochrane Clinical Trials Library, and manual searches.
A systematic review of the literature using PubMed, EMBASE, the Cochrane library and manual searches was performed until April 2008.
Ligations into pDNR-LIB (Clontech) were performed immediately following cDNA size fractionation and elution according to the method specified in the SMART cDNA library construction manual.
This study reveals that for the coverage of all isoenzymes including allelic variants represented by the cDNA library sequenced, manual work to verify the resulting transcript sequences cannot be avoided.
Object models can be acquired directly from images, allowing efficient construction of model libraries without manual intervention.
Selected phages were amplified, purified, and titrated according to the Ph.D. Phage Display Libraries Instruction Manual (New England Biolabs).
Still the PF rates as well as the >Q30%, varies as much between the lanes run with the manual libraries as between manually and automatically prepared libraries.
Mr. Seyfer and Mr. Brewster keep libraries of manuals for repairing older cars.
This paper discusses how a hybrid library (digital and manual) has been set up at the KEMRI/Wellcome Trust Research Laboratory (KEMRI/WTRL) Centre based in a rural district along the Kenyan coast.
The manual library was prepared using MID6 ("sample SPRI").
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