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Thus, the embryo library is representative of the transcriptome of embryos at different stages of development.
The head library is representative of the transcriptome of adult heads of both sexes and different physiological states (immature, virgin, mated).
Screening the library with single-copy or multi-copy gene probes showed 6 28 primary positive clones per probe of which 50 90% were true positives, demonstrating that the BAC library is representative of the different regions of the nurse shark genome.
While confirmation testing conducted on the assembly (see Confirmation of DUF1220 Copy Estimates section) indicates that the CHM1 library is representative of the general population, it cannot be ruled out that some of the changes may be true variants within the human population.
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Sequence analysis also reveals that normalization and especially subtraction were effective in decreasing redundancy, and that the pooled-tissue library was representative of the initial tissue complexity.
To maximize yield and purity and to ensure that genomic complexity of the library was representative of the original bacterial population, we defined a suitable protocol for total DNA extraction from cheese which can also be applied to other lipid-rich foods.
This approach can also be used to evaluate the extent to which microsatellite-enriched libraries are representative of the genome from which they were isolated.
Moreover, two other experimental approaches allowed us to conclude that the unequalized abundance of exogenous transcripts in our libraries was representative of the behaviour of endogenous transcripts.
Collectively, this data shows that the mRNA isolated to construct the photoperiod and hormone libraries are representative of broad temporal and induced gene expression patterns.
We assessed the extent to which enriched libraries were representative by comparing simulated and observed indicators: distribution on the chromosomes, proportion and abundance of microsatellite motifs and the length of the sequences obtained.
MicroRNAs 20b, 206 and 133 have known expression patterns between the tissues [ 21]. qPCR analysis performed directly on the sequencing libraries for these transcripts confirmed that the miRNA libraries were representative for the biological input samples (Additional file 1 Figure S1).
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