Sentence examples for library culture from inspiring English sources

Moreover, pertinent stakeholders should promote library culture through the issuance of and enforcement of standards.

The town features a hospital, primary and secondary school, university, sports center with a swimming pool, library, culture center, cinema, bus transport, hotels, a bank, and several museums.

One of the M. aquaeolei library cultures and the control were stressed with 0.05 % pinene (v/v), while the other M. aquaeolei library culture received no exposure to pinene.

After 8 hr, the library culture was split into six distinct environmental conditions (25°, 30°, 36°, 25° + S, 30° + S, and 36° + S, where S represents the addition of 0.5 M sodium chloride), and the cultures were grown for 12 20 generations with samples taken at distinct time points and stored at −80°.

In order to establish a cDNA library culture, the entire transformation reaction was used to inoculate Luria Bertani broth (10 ml final volume) containing kanamycin (50 μg ml-1 final concentration) and incubated at 37°C with shaking (225 rpm) for 18 h.

This was accomplished by collecting non-fluorescent cells from library cultures that expressed the substrate reporters alone (only 0.2% arabinose and no IPTG was added to the cell cultures).

However, false-positive library members (i.e., clones that express reporter peptides lacking the degradation tag, ssrA, due to stop codons and/or frame-shift mutations, or members where the reporter substrate is processed by an endogenous protease) had first to be depleted from the library cultures.

Library cultures were incubated overnight at 225 rpm and 37 °C.

Pooled library cultures grown at 32° to OD600 0.6 in EMM without nitrogen were tested for spores.

For the spore library, cultures of the reference isolate SLKW1407 were grown for seven days on 1% MEA.

Plasmid DNA of VH and VL library cultures was isolated with Pure Yield™ Plasmid Midiprep System from Promega.