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We have developed CoESPRIT, a modified version of the ESPRIT random library construct screen used previously on single proteins, to express soluble protein complexes.
The resulted pPF-Lib-p3 library construct is illustrated in Figure 1B and C. All steps of the phage display selection and analysis of individual peptide-displaying clones were carried out as previously described [70].
pLP1238 (NAB3 in pRS202) and pLP2018 (NAB3 in pRS426) were subcloned from pLP1419 (NAB3 library construct) using EcoRI and XhoI.
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A 14-kb clone was isolated from a flounder genomic library constructed in λFIXII.
The inner dome of the Soneva Kiri library, constructed from bamboo.
The BAC library constructed by Matsuda et al. [29] was used for chromosome walking.
The fragments were amplified and a library constructed as described previously [36].
Panel a: Library constructed with total RNA as template.
Different from the cDNA library constructed in this study, the library constructed by Ling and colleagues may have high percentage of storage transcripts in un-germinated urediniospores.
For instance, the library constructed from Italian ryegrass root tissue contained substantially more bacterium-derived DNA than libraries constructed for any of the other species.
The cDNA library constructed by SMART technology ensured the integrity of cDNA library information [ 16, 17].
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