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The analysis of GO terms shared by pair-wise library combinations allows to determine which genes are continuously or transiently down- or up-regulated during the studied process.
These were the following library combinations: union of all genes in all normalized and non-normalized leaf libraries, union of all genes in libraries E and L and union of all genes from all obtained libraries.
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Briefly, p-values were computed to reflect the significance of the difference between two counts (n1 and n2 corresponding to any two library combination out of the six libraries) using a binominal model.
When considering two insert size libraries, combination of 5 or 8 kb and 20 or 25 kb are most optimal with N50's of ~0.5 Mb.
All NG technology and library type combinations require greater depth of sequencing to reach the same level as its traditional counterpart.
Published articles were searched for on electronic databases (Pubmed, Embase, Scopus, LILACS, African Index Medicus, Cochrane Library), using combinations of the following key words: ("surveillance" OR outbreak detection OR warning system) AND (over-the-counter OR "prescription drugs" OR pharmacy OR (pharmaceutical OR drug OR medication) sales).
RNA-Seq analyses typically start by mapping sequencing reads onto the reference genome, transcript libraries, exon-exon junction libraries, or combinations thereof.
Our data demonstrate that the use of random peptide phage display to select disease-specific mimotopes from a large library in combination with computational analysis may lead to the identification of novel immunogens.
Small molecule libraries and combinations of compounds have been screened to identify substitutes for transgenic DNA delivery.
This updated library in combination with RepeatMasker (http://www.repeatmasker.org/) was used for the identification of repeats and their frequencies.
The RT-qPCR assay was designed and optimised to be monitored using hydrolysis probes from the Universal Probe Library (UPL) library in combination with gene-specific primers [ 31, 32].
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