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A third assembly (L1L2) was completed using the reads from both libraries to avoid sequence redundancy when developing SSR markers.
Root RNA was not included in these libraries to avoid potential contamination from mycorrhizal fungi which are not easy to be removed from the root tissue.
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The boy said he was "embarrassed" and "intimidated" by the incident and now spends lunchtimes in the library to avoid being teased by classmates who have seen the image.
A single candidate mutation was considered only once for each dbEST library, to avoid bias due to RNA copy number.
Therefore, when results of a serial of pairwise comparisons are to be compared, we normalize N1, N2, x and y to the smallest library to avoid the influence of different sampling size among different library pairs.
Throughout the library preparation, care was taken not to vortex or vigorously pipette/mix the library to avoid shearing the DNA into smaller fragments.
In expression profiling it is preferable to use sequences from a non-normalized cDNA library to avoid bias in the estimates of expression individual genes [ 49].
At SNP sites in each assembly, we randomly selected one read from each library to avoid multiple sampling of the same allele in the same individual.
However, this result might be explained by the removal of A. aegypti TE references from the library to avoid any bias toward this species in the annotation, which might have removed elements specific to the Aedes genus.
However, our analysis suggests that only a single BAC mate pair link should be allowed from any one plate in the BAC library to avoid the high rate of apparently the same clone on the same plate contributing to the generation of incorrect links.
KEN AULETTA: By agreeing to allow Google to undertake the expense of digitizing their collections, some of our foremost libraries hope to avoid becoming irrelevant.
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