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Until recently, four BAC libraries of barley have been published.
In this paper we report on the development and characterization of a set of five new publicly available BAC libraries of barley cultivar Morex - a cultivar selected by the International Barley Sequencing Consortium as reference genotype for genome sequencing [ 21].
Information about gene models for all these proteins is compiled in Tables I, III and IV from Additional file 3. C13, cysteineeine proteinases and cystatin sequences were used to search for ESTs or cDNA sequences in publicly available libraries of barley using the tblastn program at the DNA Data Bank of Japan [ 67] and the Okayama University Barley Database [ 68].
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Five new BAC-libraries of barley cultivar Morex were constructed (see Table 1).
Based on ESTs generated from four cDNA libraries of developing barley seed tissues [ 7] a 12K unigene set was developed for barley [ 8].
Similarly, many of the differences in our findings to those of computational searches of barley EST-library published recently [ 28] can be explained by differing definitions of what constitutes a 'known' miRNA, filtering of repetitive sequences and sequences not included in the relevant version of miRBase [ 28].
Full-length cDNA clones were isolated from a Lambda ZAP Express cDNA library [52], made from epidermal strips of barley leaves, cultivar Manchuria, 14 16 h after inoculation with Bgh isolate Race I [51].
Altogether the libraries represent >25-fold the haploid genome of barley.
Due to the lack of sequenced BAC clones that represent the entire mitochondria of barley, contamination of BAC libraries by mitochondrial DNA was not determined.
Two cDNA libraries of the drought-stressed wild barley accessions were sequenced with 454 sequencing technology.
Together with the previously published BAC library of Yu et al. (2000) [ 29], more than 25-fold combined haploid genome coverage is available now in BAC libraries of the six-rowed malting barley cultivar Morex (Table 1).
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