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In this paper, we investigate the Casacore Table Data System (CTDS) used in the casacore and CASA libraries, and methods to parallelize it.
Methods that introduce posttranslational modifications in a general, mild, and non-sequence-specific manner using biologically produced peptides are complementary and in some cases conceptually preferable as they can take advantage of rapid and inexpensive access to libraries and methods to screen them.
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A corresponding full-length cDNA was isolated from X. laevis unfertilized egg and tadpole libraries (Materials and Methods).
All identified peptides were used to build the HLA allele-specific peptide assay libraries ('Materials and methods').
All annotated peptides identified from the 14 PBMC samples were then used in SpectraST (Lam et al., 2008) to build the HLA class I allele-specific peptide spectral libraries ('Materials and methods').
The key steps in this development approach are task analysis, method selection (from a library), and method configuration.
Specifically, synthetic protocols to obtain nucleosome libraries and analytical methods to read out the desired biochemical properties of the library members need to be implemented.
Nevertheless, maintenance of large mutant libraries and selection methods are difficult to implement in more complex systems such as mammalian cells and multicellular organisms.
Overall, we have established an improved framework for the design and implementation of RNAi screens using currently available libraries and analysis methods.
note: 1. "Charles Manson and the Manson Family," Marilyn Bardsley, on Crime Library: Criminal Minds and Methods, http://www.crimelibrary.com/serial_killers/notorio... Sign up for the best of VICE, delivered to your inbox daily.
We carried out a screening campaign using a small collection of 640 compounds selected as representative of the ETP-CNIO library (Materials and Methods).
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