Biotinylated PfBCCP-79 was purified by affinity chromatography, and free biotin was liberated using acid hydrolysis.
Briefly, samples were fortified with stable isotope analogs of the target analytes, and glucuronide or sulfate-bound metabolites were liberated using an enzyme hydrolysis.
Adherent cells were liberated using GIBCO™ cell dissociation buffer enzyme free (Invitrogen) and resuspended in Kreb's Ringer phosphate (KRP) buffer (PBS containing 1 mM CaCl2, 1.5 mM MgCl2, 5.5 mM glucose, pH 7.4).
For example, the hydrolysis by endo-1,4-β-mannanase from Aspergillus niger BK01 showed that mannobiose was the main product and mannose was not liberated using locust bean gum as substrate [ 5].
Sucrase activity was determined by measuring the glucose liberated using glucose kits (Merck, Germany, Cat # 1.16720.0001), while levan formed was measured using the following procedure: 500 μl of the sample was added to 750 μl ethanol (to precipitate levan), and then the mixture was centrifuged.
The obvious benefit: rather than being obligated to sit in front of the defense, Jones was liberated to use one of his prime virtues, his ability to cover ground, to have a greater influence.
For decades the gender pay gap has remained stubbornly persistent, and I hope that women now feel liberated to use the legislation to improve their pay and other conditions at work – regardless of their sector, industry or workplace.
Of course, not all the participants are total chubsters (a term I feel liberated to use in the delightfully retro context of the darts) but quite a few do look more corpulent than ever this year.
For time course 2, we lysed packed RBCs using a.05%% saponin solution, washed liberated parasites using phosphate-buffered saline (pH 7.4), pelleted parasites at 13.2 RPM in a micro-centrifuge, and stored parasites in 1 mL of TRIZOL reagent at −80 °C prior to RNA extraction.
Over 3 decades ago a correlation was described between the neuraminidase activity in the middle and distal small intestine and the milk Sia content that led the authors to suggest that milk Sia is liberated and used by the suckling newborn [18].
