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The liberated phenol is measured colorimetrically in the presence of 4-aminophenazone and potassium ferricyanide at wavelength of 510 nm.
The liberated phenol couples with the hexazotized pararosaniline solution to give an insoluble, brightly colored azo dye seen as pink bands in the gel.
Similar(58)
Then the activity of acid phosphatase was calculated as mM phenol liberated per mg of protein after 30 min of incubation at 37°C using suitable standard curve.
The activity of alkaline phosphatase was calculated as mM phenol liberated per mg of protein after 30 min of incubation at 37°C using suitable standard curve.
The lipase assay was performed spectrophotometrically using p-nitro phenyl palmitate as substrate [ 31]. p-nitro phenol was liberated from p-nitro phenyl palmitate by lipase mediated hydrolysis.
Phenolates can also be liberated from 216.
I felt wildly liberated.
Now he is liberated.
She will be liberated.
" 'Somalia has been liberated!
I'm liberated.
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