The meditator is guided by an intention to self-regulate, to self-explore, and finally to self-liberate using an attentive moment-by-moment focus on the contents of consciousness with an attitude of compassionate open-heartedness.
Biotinylated PfBCCP-79 was purified by affinity chromatography, and free biotin was liberated using acid hydrolysis.
Briefly, samples were fortified with stable isotope analogs of the target analytes, and glucuronide or sulfate-bound metabolites were liberated using an enzyme hydrolysis.
Adherent cells were liberated using GIBCO™ cell dissociation buffer enzyme free (Invitrogen) and resuspended in Kreb's Ringer phosphate (KRP) buffer (PBS containing 1 mM CaCl2, 1.5 mM MgCl2, 5.5 mM glucose, pH 7.4).
For example, the hydrolysis by endo-1,4-β-mannanase from Aspergillus niger BK01 showed that mannobiose was the main product and mannose was not liberated using locust bean gum as substrate [ 5].
Sucrase activity was determined by measuring the glucose liberated using glucose kits (Merck, Germany, Cat # 1.16720.0001), while levan formed was measured using the following procedure: 500 μl of the sample was added to 750 μl ethanol (to precipitate levan), and then the mixture was centrifuged.
The final step detected liberated H2O2 using DAOS [ N-ethyl N- 2-hydroxy-3-sulfopropyl -3,5-dimethoxyaniline N- 2-hydroxy-3-sulfopropyl -3,5-dimethoxyaniline N- 2-hydroxy-3-sulfopropyl -3,5-dimethoxyaniline N- 2-hydroxy-3-sulfopropyl -3,5-dimethoxyaniline N- 2-hydroxy-3-sulfopropyl -3,5-dimethoxyaniline N- 2-hydroxy-3-sulfopropyl -3,5-dimethoxyaniline N- 2-hydroxy-3-sulfopropyl -3,5-dimethoxyaniline
Our objective is to liberate restaurants from using manual booking systems and costly competitors.
Even though Guadagnino says that La Piscine was not exactly his "cup of tea", he found it "liberating" to use it as the basis for a new film.
The obvious benefit: rather than being obligated to sit in front of the defense, Jones was liberated to use one of his prime virtues, his ability to cover ground, to have a greater influence.
For decades the gender pay gap has remained stubbornly persistent, and I hope that women now feel liberated to use the legislation to improve their pay and other conditions at work – regardless of their sector, industry or workplace.
