In the case of LF, both His and the native N-terminal residue, Ala, did not have a destabilizing effect and behaved similarly to the LF construct with the stabilizing Gly residue, however, they behaved differently in cell toxicity experiments.
As predicted by the N-end rule, the LF protein with the stabilizing Gly N-terminus was comparable to a LF construct with native wild type amino terminus (Ala) in cell toxicity studies and the rat lethality model.
LF constructs having the N-end rule destabilizing residues Arg and Phe, however, showed higher average TTD of 78 min and 122, respectively (Table 2).
Furthermore, LF constructs with the N-end rule stabilizing residues Met or Gly, and the intermediate stabilizing residues, His and Ala, resulted in a similarly low TTD (Table 2).
The copula is an ideal tool to combine two marginal univariate LF to construct a bivariate LFs.
Then by taking the constructed LF metamodel as a prior-knowledge and mapping the output space of the LF metamodel to that of the studied high-fidelity (HF) model, a variable fidelity (VF) metamodel is created to approximate the relationships between the design variables and corresponding output responses.
The test areas as shown in Figure 1, namely the standard (ST), cattle manure (CM), and pig liquid fertilizer (LF) areas, were constructed in 5 × 10-m sites on an empty farmland in Korea (36°19′45.71″ latitude and 127°37′16.91″ longitude).
When the corresponding HF response is gained, a function that maps the LF outputs to HF outputs is constructed via LSSVR.
The potential of Thermal Solid Sample Introduction (TSSI Fast Gas Chromatography (GC Low Flow Ion Mobility Spectrometry (LF-IMS) having been designed and constructed in Engineering Research Center of Esfahan, detector group was investigated for chemical detection capabilities.
Since the entropy per dimension l is logarithmically proportional to the number of equal-probable intervals S (or lfs & Sfs for our approach) constructed in each dimension, this can be written as l = L ∕ D = log 2 S for typical EP discretization scheme (9).
