Protective antigen (PA) and edema factor (EF) combine to form edema toxin (ET) and PA and lethal factor (LF) combine to form lethal toxin (LT).
In the RIDA-LF combined assay, chemically labeled probes are covalently conjugated to magnetic microbeads, which is propitious to separate cleaved probes from the reaction solution.
The real-time assay shows that RIDA is able to specifically detect target sequences in 5 to 15 min. The RIDA-LF combined assay can specifically detect nucleic acid targets without sophisticated equipment.
Although we have previously demonstrated that systemic LF treatment combined with T-cell modulation can selectively expand tolerogenic DC in a transplantation model [ 14], the ability of tolerogenic DC generated in vitro to serve as an antigen-specific tolerogenic tool has not been shown.
The HF residue was re-suspended twice in fresh NaI solution, and the LFs were combined.
JS, LF, and BS combined and modified existing micro-scale tools into MAPS, participated in study design and data collection and management, and edited the manuscript.
The combined H LF-NMR T2 distributions of saturated and unsaturated FAMEs are presented in Figs. 4a e and 5a c with increasing chain length and degree of unsaturation, respectively.
LF-MF (0.5-16.5 Hz) combined with a static MF could markedly suppress tumor growth in Ehrlich ascites carcinoma (EAC) model mice [ 2].
The combined H LF-NMR T2 distributions of oleic acid (OA) and methyl oleate (MO) at different temperatures are presented in Figure 1A and B, respectively.
Throughout this manuscript, FAMEs are identified by their structures.> -wrap-foot> T m melting temperature The combined H LF-NMR T2 distributions of some saturated and unsaturated FAMEs at 313 K are presented in Fig. 1.
The ultrastuctural alterations caused by Lf to the bacteria enhance the activity of some antimicrobial agents [ 7, 23], and one approach could be to combine Lf with antibiotics in treating infections.
