Another human colon cancer cell line adapted to LF conditions, WiDr LF, displayed only a moderate increase in the constitutive expression of BCRP protein and mRNA levels (1.6-fold) compared with their HF counterpart, as revealed by western blot and real-time LC-PCR.
Besides, glare is reduced when the background luminance increases (Lf), considering that the observer has his vision adapted to this value.
To cross-culturally adapt the Short Form of the McGill Pain Questionnaire (SF-MPQ) into Brazilian-Portuguese and test the clinimetric properties of the newly developed SF-MPQ and the previously cross-culturally adapted Brazilian-Portuguese Long Form of the McGill Pain Questionnaire (LF-MPQ).
No differences on gefitinib sensitivity were observed between the WiDr HF- and LF-adapted cell lines.
Consistently Caco-2 and WiDr HF- and LF-adapted cells displayed a similar intracellular localisation of BCRP.
In WiDr HF- and LF-adapted cells EGFR protein expression was markedly lower than in Caco-2 cells.
In the present study, we investigated whether erlotinib resistance in the LF-adapted cells could be associated with a decrease or loss of EGFR protein.
The BCRP C421A polymorphism was studied in Caco-2 and WiDr HF- and LF-adapted cells as well as in MCF-7/MR.
For both WiDr and Caco-2, the levels of pAkt were similar in HF- and LF-adapted cells, suggesting that cellular folate status has no impact in the phosphorylation status of Akt.
Here we show that these LF-adapted cell lines had a 1.3 2.2-fold 1.3 2.2-foldthe constincreaseBCRP protein expression along withe3- to 4-fold inconstitutivee mRNA levels.
To investigate whether the different levels of BCRP expression in the Caco-2 and WiDr HF- and LF-adapted cell lines would have an impact in the anticancer efficacy of gefitinib and erlotinib, we performed growth inhibition studies in these cells as well as in the BCRP-overexpressing cell line MCF-7/MR.
