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The sequenced reads were clipped one nucleotide in both ends, leaving 74 nucleotide long reads for sequence alignment to human reference genome (GRCh38/hg38) by using the STAR software51.

The ancestral alleles were determined by whole-genome sequence alignment to 14 other avian species.

Traditionally, people use pairwise sequence alignment to detect homology between proteins.

(a) Partial primary sequence alignment (from the end of S6 to the beginning of the A helix) among Kv7 subunits.

In sequence alignment, we want to associate residues in a query sequence with homologous residues in a target database sequence.

Sequence homology of IS3 cDNA between human and murine genomes by ClustalW2 Multiple Sequence Alignment is known to be 88.35%.

Chang, J. M., Di Tommaso, P. & Notredame, C. TCS: a new multiple sequence alignment reliability measure to estimate alignment accuracy and improve phylogenetic tree reconstruction.

The number of sequences per multiple sequence alignment varied according to domains, with a median of 12 sequences per alignment.

One way to leverage sequence data is to use genome-scale metabolic models.

a, Multiple sequence alignment of mammalian Cx46 and Cx50 isoforms with residues contributing to heteromeric and heterotypic interfaces annotated83.

A multiple sequence alignment of all sequences was generated using MUSCLE22, and the resulting alignment file was used to generate a maximum-likelihood tree with FastTree23.

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