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Extra- and intracellular lactate levels were quantified using methods reported by us.
Protein levels were quantified using a Pierce ™ BCAprotein assay kit according to the manufacturer's recommendations.
The uCTXII levels were quantified using the Urine Pre-Clinical Cartilaps ELISA (Immunodiagnostic Systems, USA).
JP8 exposure levels were quantified using both personal air monitoring and urinary biomarkers of exposure.
Brain nuclei were micro-dissected and GAD65 protein levels were quantified using western immunoblotting.
Read buffer T was then added, and the cytokine levels were quantified using a Sector Imager 6000.
Soluble protein levels were quantified using a BioRad Bradford assay.
Haematocrit levels were quantified using the Sysmex SC9500 analyser (Japan).
FFA levels were quantified using an enzymatic colorimetric assay (NEFA C) (Wako Chemicals GmbH).
Specific mRNA levels were quantified using the threshold method [53] and normalized to GAPDH (ΔCT values).
Relative TIEG1 and Runx2 protein levels were quantified using Zeiss LSM 510 software.
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