TG levels were measured using assay kits from BioVision.
Depression levels were measured using several common psychological scales.
Fitness levels were measured using a type of treadmill test.
CCL7 levels were measured using an ELISA kit (Origene, Rockville, MD and Innovative Research, Novi, MI).
RNA samples underwent reverse transcription and cDNA levels were measured using target-specific primers.
Serum BDNF levels were measured using an ELISA kit (BiosensisRapidTM Mature BDNF).
Glucose and triglyceride levels were measured using Accutrend Technology (Roche Diagnostics, Basel, Switzerland).
Maternal serum BDNF levels were measured using a competitive enzyme-linked immunosorbent assay (ELISA).
Serum creatinine levels were measured using commercial assay kits according to the manufacturer's instructions (K625 100, Biovision, Milpitas, CA, USA).
Serum corticosterone levels were measured using Corticosterone ELISA kit (Abcam LLC, Cambridge, UK) in accordance with manufacturer's instructions.
Plasma Cathepsin B levels were measured using an ELISA Kit (Abcam Human Cathepsin B) following the manufacturer's instructions.
