Intra-group differences in plasma FDG and glucose levels were evaluated using the Student's t test.
Intracellular calcium levels were evaluated using a FURA-2/wire myograph system.
Finally, the effects of plant scale factors on impact levels were evaluated using grey model GM (1, N) too.
Gene expression of FGF2 and VEGFA on the mRNA and protein levels were evaluated using quantitative polymerase chain reaction and enzyme-linked immunosorbent assay methods.
Legal impact Both the indications of use for all the surgical procedures, categories of patients, and presence of authorization of use at national, European and International levels were evaluated using a 7-item Likert scale.
Anxiety levels were evaluated using the EPM test [44].
Tumor vasculature and immune cell infiltration were evaluated using immunohistochemistry. Cytokine levels were evaluated using ELISA and electrochemiluminescence.
Intracellular cAMP levels were evaluated using a direct cAMP enzyme immunoassay (Assay Designs, Ann Arbor, MI), per the manufacturer's recommendations, including optional acytelation of samples.
In BV2 cells treated with 0.1, 1 and 10 µM CT peptides or 1 and 10 µM Aβ peptides for 48 h, [Ca2+]i levels were evaluated using the Fluo3/AM method.
Relations between recognition score (calculated as the difference between the investigation time for the novel and the familiar stimulus) and c-Fos levels were evaluated using Bravais-Pearson's correlation tests.
Serum estradiol levels were evaluated using a human estradiol ELISA kit; Fertigenix-E2-EASIA (Biosource Europe S.A, Nivelles, Belgium) (no significant difference between men and women, data not shown).
