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Protein levels were evaluated by western blot using anti Nf2 (sc-332) and anti-Bap1 (Sc-28383) antibodies (Santa-Cruz).
The thermal and shear stress levels were evaluated by a cooling index and the wall shear stress.
The cellular glutathione (GSH) content, superoxide dismutase (SOD) activity and malonaldehyde (MDA) levels were evaluated by spectrophotometer.
The protein levels were analyzed by Western blot and the mRNA levels were evaluated by real-time PCR.
Serum interleukin (IL -1β, IL -1βnd tumor necrosis factor (TNF)-α levels were evaluated by enzyme-linked immunosorbent anday (ELISA).
Samples were subjected to agitation stress, and soluble aggregate levels were evaluated by size-exclusion chromatography and verified with analytical ultracentrifugation.
HPD levels were evaluated by the Personality Disorder Questionnaire-4 and Axis I symptoms were controlled for using the brief symptoms inventory.
Serum interleukin (IL -1, IL -1 and tumor necrosis factor (TNF)-α levels were evaluated by enzyme-linked immunosorbent anday (ELISA).
Maternal alexithymia levels were evaluated by TAS-20.
Differences in PtdIns5P levels were evaluated by the Mann-Whitney (U -test [23].
RNA levels were evaluated by OD measurement at 260 nm on a Nano-drop 2000 (Thermo Scientific, Wilmington, DE).
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