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The LUMO energy levels were estimated using the optical bandgap.
Ethanol levels were estimated using chromatographic techniques, such as HPLC and GC.
IL-1β levels were estimated using human IL-1β ELISA kit Diaclone-Eli-pair (Besancon, France).
The number of quantization levels was 8 for the whole database and the quantization levels were estimated using the Linde-Buzo-Gray (LBG) vector quantization algorithm [22].
Briefly, expression levels were estimated using GCRMA [26].
Images were acquired and expression levels were estimated using a Zeiss 510 confocal microscope.
Intracellular superoxide anion levels were estimated using the fluorescent dye dihydroethidium (DHE), obtained from Sigma-Aldrich.
Kermit 2 morpholino sequence: AGAGGCATCTTTCTTTCAGCGAAGG. Kermit2 5 place mis-match morpholino: AGAcGCATgTTTgTTTCAGCcAAcG. Gene expression levels were estimated using RT-PCR.
Relative transcript levels were estimated using the standard curve method described previously by Dale et al. [74].
The relative HNF4α RNA expression levels were estimated using the ΔΔCt method with normalization to mouse GAPDH RNA.
Gene expression levels were estimated using RSEM v1.2.12 [ 43].
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