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EGFR expression levels were confirmed using western blotting (Fig. 1c).
Their expression levels were confirmed using immunoblotting against His-tag (Fig. S2A).
Protein levels were confirmed using 2-DE, immunoblotting and immunohistochemistry.
Exposure levels were confirmed using high performance liquid chromatography as described previously [ 42].
The effects of GSH depletors on intracellular GSH levels were confirmed using the GSH reductase-DTNB recycling method.
Low cell toxicity levels were confirmed using Cytotox 96 kit (Promega, Madison, WI) from culture supernatants according to manufacturer's instructions.
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The effect of ectopic SOX2 on BIM protein levels was confirmed using two different expression strategies.
A role for Trx1 downregulation in the observed increase in ROS levels was confirmed using Trx1-specific siRNA.
The OCC presence and level were confirmed using a GC mass spectrometry system (VG Trio 2; VG Analytical, Manchester, UK) in randomly selected samples.
A reduction in the L11 protein level was confirmed using a rabbit polyclonal antibody specific to human L11 [19].
Also, the protein levels of SOCS3 were confirmed using Western blotting (Figure 4I).
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