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Expression levels were calculated using the relative quantification method.
Relative expression levels were calculated using 2−△△Ct method2−△△Ct
The relative expression levels were calculated using the 2−ΔΔCt method2−ΔΔCt
Relative expression levels were calculated using CFX Manager 3.1 (Bio-Rad) software and the ΔΔCT method23.
LDL cholesterol levels were calculated using the Friedewald equation.
Ratios of ipsilateral-side mRNA levels to contralateral-side mRNA levels were calculated using the ΔCt method (2−ΔΔCt).
The electronic structures and energy levels were calculated using the B3LYP/6-31G B3LYP/6-31Gt.
The relative mRNA levels were calculated using the comparative Ct method, using the house keeping genes β-actin and GAPDH as an internal control.
Gene expression levels were calculated using FPKM values.
mRNA levels were calculated using the 2−ΔΔCT method.
Relative gene transcript levels were calculated using the 2-ΔΔCT method.
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