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Kiss1 mRNA levels were assessed using an in situ hybridization (ISH) protocol described by us previously [ 22, 30].
One week after the first dose, blood glucose levels were assessed using a glucometer.
The correlations of the two HbA1c levels were assessed, using Pearson's correlation coefficient.
Plasma PCSK9 levels were assessed using ELISA at the end of each phase.
SAP levels were assessed using a validated ELISA method, non-discriminating between endogenous and exogenous SAP.
Their pain levels were assessed using the Visual Analogue Scale (VAS).
The 3243A>G heteroplasmy levels were assessed using RFLP after the sub-clones were expanded for one week.
Thus, in order to further test the low toxicity of MoS2, PEGylated MoS2, and MoS2-PEG-PEI, intracellular ROS levels were assessed using a dihydroethidine (DHE) probe.
Superoxide (O2−) levels were assessed using the fluorescent probe, HE.
ATP levels were assessed using an ATP bioluminescence assay kit (Roche) [13].
RNA levels were assessed using phosphorimaging techniques and Image Quant software (version 2.4).
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