Sentence examples for levels were assayed using from inspiring English sources
IL-6 and IFNγ levels were assayed using ELISA preoperatively and at 24 and 48 hours.
Luciferase levels were assayed using the Dual Luciferase Assay (Promega, Madison, WI) following manufacturer's instructions.
Plasma TG and cholesterol levels were assayed using commercially available kits (Vitros or Chema Diagnostica, Iesi, Italy).
VEGF protein levels were assayed using a human VEGF-specific ELISA Kit (Quantikine; R&D Systems, Minneapolis, MN) and measured by a microplate reader set to 450 nm.
Total cellular ATP levels were assayed using the CellTiter-GLO® Luminescent Cell Viability kit (Promega), according to the manufacturer's recommendations and as previously described [20].
AT1R mRNA and protein levels were assayed using quantitative polymerase chain reaction (qPCR) and western blotting (WB), respectively, after intracerebroventricular (ICV) administration of Ang II.
Plasma LH level was assayed using a highly specific rabbit anti-serum, anti-native rat LH (ALPCO diagnostics # 29-R002) wI125I125 as tracer.
IL-22 cytokine level was assayed using a commercially available IL-22 ELISA kit (R&D Systems) according to manufacturers' instructions.
Serum vitamin (25 (OH)D3 level was assayed using ELISA method (Cusabio, normal value>30 ng/mL).
HbA1c level was assayed using high-performance liquid chromatography (HPLC), which is recommended by the National Glycohemoglobin Standardization Program [ 16].
Serum ALP level was assayed using a commercial kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) with a spectrophotometer (Shimadzu, Kyoto, Japan).
