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The Otsu algorithm failed here, confusing the outer layer and the brighter insets with one another, so manual selection of threshold levels was used on this image by visually comparing the result with the source phantom a procedure which is obviously impossible with real data.
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The total DNA levels were used to assay cell adhesion on day 1, and cell proliferation during the 7-day culture period.
A confidence level of 95% was used on all analyses.
Study-level binomial regression was used on the pooled data across the included studies.
The p-value was obtained using 999 Monte Carlo simulations and a 5%% significance level was used based on a likelihood ratio test.
Obstructive lesions on a segment level were used to determine obstructive CAD on a patient level as having one or more obstructive stenoses (≥50 % lumen diameter stenosis) irrespective of the segment in which they were located.
Stratified random sampling methods based on referral level are used for the hospital survey on PHE preparedness because it is found that the preparedness level depends on the referral level [ 14].
A purposive sampling technique, with predefined criteria, was used on all levels and snowball sampling was used at the community level.
Wistar expression levels were used as calibrators in qPCR on fresh islets, while Wistar expression level at 2.8 mM glucose were used as calibrators in qPCR on GSIS experiments.
For instance, point mobility calculations on subcomponent level are used to obtain more accurate input parameters for statistical energy analysis (SEA) models.
In this study, the HA genes of five different clades with 95 to 99% identity on protein level were used to analyze cross protection in a mouse model.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com